Curriculum Vitae



Duquesne University (August, 1993 - October, 1999)
Ph.D. in Analytical Chemistry awarded in October, 1999.
Advisor: Mitchell Evan Johnson, Ph.D.
Thesis Title: “Strategies for Near-Infrared Diode Laser-Induced Fluorescence Detection of Bioactive Analytes in Capillary Electrophoresis”.

University of Pittsburgh (August, 1989 - June, 1993)
B.S. in Chemistry awarded August, 1993
Advisor: Andrew G. “Jack” Sharkey, Ph.D.
Undergraduate research in the use of silyl derivatization for GC-MS analysis of environmental pollutants. 


Dr. Gallaher is an Analytical Chemist by training. His doctoral research focused on the separation and detection of biologically relevant analytes at ultratrace levels using capillary electrophoresis and laser-induced fluorescence detection. He is also interested in the development of novel separation and detection methodology for analytes of biological and environmental significance. Dr. Gallaher received a B.S. in Chemistry from the University of Pittsburgh in 1993, followed by his Ph.D. from Duquesne University in 1999. He has published scientific papers in several chemistry journals including, Analytical Chemistry, Applied Spectroscopy, and The Analyst. Dr. Gallaher conducted post-doctoral research at Duquesne University examining the high-temperature auto-oxidation of jet fuels and the tannin content of western Pennsylvania white oak trees used in the production of wine barrel staves. Since arriving at Carlow in the Fall of 2000, Dr. Gallaher ha s taught a wide variety of courses at various levels, including lectures and laboratory courses in Organic and Analytical Chemistry as well as Junior Seminar and Senior Experience. He has served as the faculty Advisor to the Carlow University Chapter of the American Chemical Society Student Affiliates since 2001. Dr. Gallaher has written and received several grants during his tenure at Carlow, which have been used to upgrade and expand the chemical instrumentation capabilities of the Chemistry Department and increase hands-on learning opportunities for the students. Dr. Gallaher is currently serving his second tour as Departmental Chairperson and holds the rank of Associate Professor of Chemistry.


1) B. Beaver, V. Sharief, Y. Teng, R. DeMunshi, J.P. Guo, E. Katondo, D. Gallaher, D. Bunk, J. Grodowski, P.  Neta; “Development of Oxygen Scavenger Additives for Future Jet Fuels. A Role for Electron‐Transfer‐  Initiated‐Oxygenation (ETIO) of 1,2,5‐Trimethylpyrrole?” Energy & Fuels 2000, 14, 441‐447    

2) Gallaher, D. L.; Johnson, M. E. 'Nonaqueous Capillary Electrophoresis of Fatty Acids Derivatized with a  Near‐infrared Fluorophore' Analytical Chemistry, 2000, 72(9), 2080‐2086.    

3) Gallaher, Jr., D. L.; Johnson, M. E. “Development Of Near‐Infrared Fluorophoric Labels For The  Determination Of Fatty Acids Separated By Capillary Electrophoresis with Diode Laser Induced  Fluorescence Detection”. Analyst, 1999, (124) 1541‐1546.    

4) Gallaher, Jr. , D.L.; Johnson, M.E. “Nonaqueous Capillary Electrophoresis of Linear Free Fatty Acids with  Near Infrared Laser‐Induced Fluorescence Detection” Presentation given at REDUCS 99’, Duquesne  University, Pittsburgh, PA (USA), July 31, 1999.    

5) Gallaher, Jr., D. L.; Johnson, M. E. “Synthesis and Characterization of Near‐Infrared Fluorophoric Labels For  The Derivatization and Diode Laser‐Induced Fluorescence Detection of Free Fatty Acids Separated by  Capillary Electrophoresis". Presented at the 31st ACS Central Regional Meeting, Fawcett Conference  Center (OSU) Columbus, Ohio; June 23, 1999.    

6) Johnson, M.E.; Gallaher, Jr., D. L. “Synthesis and Characterization of Near‐Infrared Fluorophoric Labels for  the Sensitive Determination of Biologically Important Carboxylic Acids” Presented at FACSS XXV, Austin,  Texas (USA), October, 1998.    

7) Gallaher, Jr., D. L.; Johnson, M.E. “Synthesis and Characterization of Near‐Infrared Fluorophoric Labels for  the Sensitive Determination of Biologically Important Carboxylic Acids”. Presentation given at REDUCS 98’,  Duquesne University, Pittsburgh, PA (USA), August, 1998.    

8) Gallaher, Jr., D. L.; Johnson, M. E. “Characterization of a Rugged, Open‐Gap Flow Cell for Confocal Laser‐  Induced Fluorescence Detection in Capillary Electrophoresis.” Applied Spectroscopy, 1998, 52(2), 292‐297.    

9) Gallaher, Jr., D. L.; Johnson, M. E. ‘Confocal Mode Configuration for Laser‐Induced Fluorescence Detection  in Capillary Electrophoresis.’ Poster presented at FACSS XXIV, Providence, RI (USA), October, 1997.    

10) Gallaher, Jr., D. L.; Johnson, M. E. ‘Development of a Windowless Flow Cell for the Enhancement of Signalto‐  Noise Ratio in Capillary Electrophoresis using Confocal Laser‐Induced Fluorescence Detection.’ Poster  presented at the Frederick Conference on Capillary Electrophoresis, Frederick, MD (USA); October, 1996.

Research Interests

Fluorescence Derivatization and Detection Strategies for Capillary Electrophoresis and HPLC:
Laser-induced fluorescence has been recognized as the most sensitive method of detection following separation by capillary electrophoresis. Derivatization chemistry is often required to extend the advantages of LIF to the target analyte. Fluorophore design, derivatization chemistry, and detection schemes are investigated and optimized to permit detection at ultra-trace levels.

Near-Infrared Fluorescence Labeling and Diode-Laser Induced Fluorescent Detection:
LIF detection schemes in the near-infrared region of the spectrum are an attractive alternative to visible fluorescence due to decreased backgrounds and interferences, and as a result, increased detection sensitivity. The use of high-powered diode laser sources can help provide high-sensitivity at a very low cost. The development of new fluorophores and derivatization chemistry are critical to the extension of NIR fluorescence detection to relevant analytes.

Design of Selective Stationary Phases using Molecular Imprinting Strategies:
Molecular imprinting technology is one method of generating chromatographic stationary phases which demonstrate a high degree of selectivity for a target analyte. Imprinted stationary phases have the potential to offer rapid, selective, and rugged analyses for analytes with clinical, biological, or pharmaceutical relevance.

Bioanalytical Separation Method Development:
Within this broad research category, I am most interested in developing CE and HPLC separation methodology for analytes with biological, medical, and pharmaceutical importance. Method development employing multi-dimensional separation schemes, selective stationary phases, and highly efficient/sensitive separation modes can be combined to develop analysis schemes for target analytes at trace levels in complex matrices.